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PFT-Host Interactions: A Tale of Two Toxins with Ifrah Shahi ‘13, Graduate Student, Vilcek Institute of Biomedical Sciences, NYU Langone Health.

Pore forming toxins (PFTs) are commonly produced by pathogenic bacteria, and
understanding them is key to the development of virulence-targeted therapeutic strategies. I
am investigating mechanisms of action of two types of PFTs. The first PFT is the rhamnolipid
toxin produced by Streptococcus agalactiae; results from a CRISPR/Cas9 screen with the
toxin conclude that it does not require a non-essential receptor for binding to host cell
membranes. The second PFT I'm working with is a member of the Cholesterol Dependent
Cytolysin (CDCs) family, where I'm characterizing a novel humanCD59-dependent toxin
produced by the bacteria Streptococcus tigurinis.

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